GRADUATE STUDENTS
TECHNICIAN
In 1990 the principal investigatorspent a sabbatical leave working with the “fish transgenic group” of theMinnesota University in USA with the objective to implement the transgenictechnology to the seabream species. The DNA transfer protocol bymicroinjection, was set up for this species. The short-term expression patternof several reporter genes driven by strong promoters was successful, thusallowing the use of this system to study genetic expression patterns of earlydevelopment genes of interest in Aquaculture.
Within the sameobjective of long-term genetic manipulation of S. aurata
A current line of research in collaboration with the Microbiology Department deals with exploiting natural genetic resources for natural immunity in seabream, seabass and sole, mostly related to the lisozyme and Mx antimicrobial systems. An ongoing project aims at developing biofactories for using those antimicrobials as immunostimulants
Identification of genes involved in early fishdevelopment. Funded by C.E Period: 1994- 1998
A comprehensive genetic study of cultured and wildstocks of gilthead sea bream (Sparus aurata) and geneticassessment of several related species as candidates for aquaculture. Funded by:C.E. Period 1995- 1999
Desarrollo decélulas totipotentes de dorada (Sparus aurata) para la manipulación genética dela especie. Funded by: Consejería de Industria, Comercio y Turismo (Junta deAndalucía). Period 1996
Influencia defactores genéticos y de la temperatura sobre el crecimiento de la lubina.
Assessment of procedures for the development of aeuropean standardised multi-site testing programme: Application to seabass, Dicentrarchuslabrax. Funded by: CE. Period 1998- 1999
Desarrollo demarcadores genéticos moleculares aloenzimas y microsatélites en el lenguadocultivado, Solea senegalensis.
EstudioGenético de poblaciones naturales marroquíes y españolas de Dentex dentex,Lithognathus mormyrus y Pagellusbogaraveo, mediantealoenzimas y microsatélites. Funded by: AECI. Period 2000- 2001
Biofactorías deinmunoproteínas de peces: su aplicación en enfermedades infecciosas. Funded byCICYT. Period 2003-2005.
Herramientas para un programa deselección asistida por marcadores en dorada (Sparus aurata
Amores, A.,Giles, V., Thode, G. and Alvarez, M.C. (1990). Adaptative character of aRobertsonian fusion in chromosomes of the fish Gobius paganellus
Martínez, G.,McEwen, I., McAndrew, B.J. and Alvarez, M.C. (1991). Electrophoretic analysisof protein variation and restriction length polymorphism in mitocondrial DNA ofthe European seabass, Dicentrarchus labrax L (Pisces, Moronidae). Aquaculture andFisheries Management. 22: 443-455.-
Alvarez, M.C.,Otis, J., Amores, A. and Guise, K. (1991). Short-term cell culturetechnique for obtaining chromosomes in marine and freshwater fish. Journalof fish Biology39: 817-824.
Gross, M. L.,Schneider, J F., Moav, N., Alvarez, C., S. H., Liu, Z., Hew, C. and Hallerman,E. (1992). Molecular analysis and growth evaluation of northern pike (Esox lucius
Alvarez, M.C.(1992). La transgenia como alternativa en la mejora genética de peces. Bol.Sociedad Española de Genética 2: 5-7.
Amores, A.,Martínez, G., Reina, J. and Alvarez, M.C. (1993). Karyotype C banding andAg-NOR in Diplodus bellottii (Sparidae, Perciforms). Intraindividual polymorphism involvingheterochromatic regions. Genome 36 (4): 672-675.
McKeown, B.A.,and Alvarez, M.C. (1994). Immunocytochemical and radioimmuno assay studies onnorthern pike (Esox lucius) and walleye (Stizostedion vitreum
Reina, J.,Martínez, G., Amores, A., and Alvarez, M.C. (1994). Interspecific geneticdifferentiation in Mediterranean Sparid fish. Aquaculture
Amores, A. Béjar,J. and Alvarez, M.C. (1995) . High-resolution replication bands in theAnguillifom fish Echelus myrus. Chromosome Research 3: 423-426.
Amores, A. Béjar,J. and Alvarez, M.C (1995) . Replication, C and AgNOR chromosome bandingin two Anguilliform fish species. Marine Biology
Alvarez M.C.(1995). Los peces, sistemas "modélicos" en la manipulación genética.Aulas del mar, Acuicultura. Zamora et al. Ed. Univ. Murcia 207-218.
Béjar J., BorregoJ.J. and Alvarez M.C. (1997) . Continuous cell line from the cultured marinespecies gilthead sea bream (Sparus aurata). Aquaculture
Garcia-Pozo Sand Alvarez M.C. (1998). Comparative expression of the plasmids pCMV-CAT andpCMVtk-lacZ in embryos and larvae of the seabream. CIHEAM Cahiers OptionsMediterranéennes34: 141-151.
Béjar, J., HongY. and Alvarez M.C. (1998). Development of ES cells from Sparus aurata
Martinez-Rodriguez,G., Alvarez M.C., and McAndrew, B. J. (1998). Genetic variability ofEuropean sea bass, Dicentrarchus labrax L: Data from a hatchery stock. AquacultureResearch 29:851-853.
Garcia-Pozo S., Béjar,J., Shaw M. and Alvarez M.C. (1998) . Effect of exogenous DNA microinjection onearly development response of the seabream (Sparus aurata
Alarcón, J.A.and Alvarez M.C. (1999). Genetic identification Sparidae species by isozymemarkers. Applications to interspecific hybrids. Aquaculture
Perez-Prieto,S.I., Rodriguez-Saint Jean, S., Garcia-Rosado, E., Castro, D., Alvarez, M.C.and Borrego, J.J. (1999). Virus susceptibility of the fish cell line SAF-1derived from gilt-head seabream. Diseases of Aquatic Organisms
Béjar, J., Hong,Y. and Alvarez M.C. (1999). Towards obtaining ES cells in the marine fishspecies Sparus aurata; Multipassage maintenance, characterization and transfection. Geneticanalysis and BiomolecularEngineering15: 125-129.
Alvarez M.C.(1999) . Peces transgénicos y Acuicultura: logros y perspectivas. En “Patología,Fisiología y Biotoxicología en especies acuáticas”
Hackett, P. B.and Alvarez M.C. (2000). Themolecular genetics of transgenic fish . Recent Advances in MarineBiotechnology,Vol. 4: Aquaculture. M. Fingerman and R. Nagabhushanam eds. Science Publishers,Inc.,Enfieldl (NH), USA, pp. 77-145.
Palma, J.,Alarcon, J. A., Alvarez, C., Zouros, E., Magoulas, A. And Andrade,JP. (2001). Developmental stability and genetic heterozygosity in wild andcultured stocks of gilthead seabream (Sparus aurata
Ayala, M.D.,López-Albors, O., Gil, F., García-Alcazar, A., Abellan, E., Alarcón, J.A.,Alvarez, M.C. Ramírez-Zarzosaand, G., and Moreno, F. (2001). Temperatureeffects on muscle growth in two populations Atlantic and Mediterranean of SeaBass (Dicentrarchus labrax L) . Aquaculture 202: 359-370.
Béjar, J.,Hong,Y. and Alvarez, M.C. (2002). ES cell like line from the marine fish Sparusaurata.Characterization and chimeras production. Transgenic Research 11:279-289.
Bargelloni, L.Alarcón, J.A., Alvarez, M.C., Penzo,E., Magoulas, A., Reis, C. and Patarnello,T. (2003). Discord in the family Sparidae (Teleostei): divergentphylogeographical patterns across the Atlantic-Mediterranean divide. Journalof Evolutionary Biology 16: 1149-1158.
Béjar, J., Hong, Y. andSchartl, M. 2003. Mitf expression is sufficient to direct differentiation ofmedaka blastula derived stem cells into melanocytes. Development
Alarcon J.A,Magoulas, A., Georgakopoulos A. Zouros E. and Alvarez, M.C. (2004).Genetic comparison of wild and cultivated european populations of the giltheadseabream (Sparus aurata ). Aquaculture 230: 65-80.
Porta, J. and Alvarez, M. C.Development of microsatellites from senegal sole (Solea senegalensis